DNA

Part:BBa_K4342008:Design

Designed by: Keaton Brown   Group: iGEM22_Austin_UTexas   (2022-10-04)


cymR YFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This YFP is controlled by a promoter recognized by the CymR repressor. The 5' AND 3' end of this part have bsa1 restriction sites that are designed to ligate to the AcrB up and AcrB downstream homologies.


Source

Marionette strains


References